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menin c terminal epitope  (Bethyl)


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    Structured Review

    Bethyl menin c terminal epitope
    Figure 9. Reduced C-menin expression in MEN1 CKO post-behavior testing. (A) WB of the mouse hippocampal protein samples with β-Tubulin antibody (loading control) showing the expression of β-Tubulin in controls, and CKO showing relatively similar fluorescence across the controls and CKO at the expected molecular weight of 50 kDA; (B) WB of the mouse hippocampal protein samples with menin C-terminal <t>epitope</t> antibody for the detection of the C-terminal fragment in the controls (C) and the conditional knockout (CKO) animals at the expected molecular weight of 20 kDA, and the bands from the control animals have higher fluorescence compared to the CKOs; (C) summary data, showing the comparison of menin-to-β-Tubulin ratio in controls and the CKOs, with a reduction in the C-menin-to-β-Tubulin ratio in the CKO. Data represent the mean ratio ±SEM. n = 4. Statistical significance (Mann–Whitney U test), ** p < 0.05.
    Menin C Terminal Epitope, supplied by Bethyl, used in various techniques. Bioz Stars score: 95/100, based on 221 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/menin+c+terminal+epitope/Menin+Antibody/pm36552783-122-2-5
    Average 95 stars, based on 221 article reviews
    menin c terminal epitope - by Bioz Stars, 2026-11
    95/100 stars

    Images

    1) Product Images from "Selective Menin Deletion in the Hippocampal CA1 Region Leads to Disruption of Contextual Memory in the MEN1 Conditional Knockout Mouse: Behavioral Restoration and Gain of Function following the Reintroduction of MEN1 Gene."

    Article Title: Selective Menin Deletion in the Hippocampal CA1 Region Leads to Disruption of Contextual Memory in the MEN1 Conditional Knockout Mouse: Behavioral Restoration and Gain of Function following the Reintroduction of MEN1 Gene.

    Journal: Cells

    doi: 10.3390/cells11244019

    Figure 9. Reduced C-menin expression in MEN1 CKO post-behavior testing. (A) WB of the mouse hippocampal protein samples with β-Tubulin antibody (loading control) showing the expression of β-Tubulin in controls, and CKO showing relatively similar fluorescence across the controls and CKO at the expected molecular weight of 50 kDA; (B) WB of the mouse hippocampal protein samples with menin C-terminal epitope antibody for the detection of the C-terminal fragment in the controls (C) and the conditional knockout (CKO) animals at the expected molecular weight of 20 kDA, and the bands from the control animals have higher fluorescence compared to the CKOs; (C) summary data, showing the comparison of menin-to-β-Tubulin ratio in controls and the CKOs, with a reduction in the C-menin-to-β-Tubulin ratio in the CKO. Data represent the mean ratio ±SEM. n = 4. Statistical significance (Mann–Whitney U test), ** p < 0.05.
    Figure Legend Snippet: Figure 9. Reduced C-menin expression in MEN1 CKO post-behavior testing. (A) WB of the mouse hippocampal protein samples with β-Tubulin antibody (loading control) showing the expression of β-Tubulin in controls, and CKO showing relatively similar fluorescence across the controls and CKO at the expected molecular weight of 50 kDA; (B) WB of the mouse hippocampal protein samples with menin C-terminal epitope antibody for the detection of the C-terminal fragment in the controls (C) and the conditional knockout (CKO) animals at the expected molecular weight of 20 kDA, and the bands from the control animals have higher fluorescence compared to the CKOs; (C) summary data, showing the comparison of menin-to-β-Tubulin ratio in controls and the CKOs, with a reduction in the C-menin-to-β-Tubulin ratio in the CKO. Data represent the mean ratio ±SEM. n = 4. Statistical significance (Mann–Whitney U test), ** p < 0.05.

    Techniques Used: Expressing, Control, Molecular Weight, Knock-Out, Comparison, MANN-WHITNEY

    Related Articles

    Expressing:

    Article Title: Selective Menin Deletion in the Hippocampal CA1 Region Leads to Disruption of Contextual Memory in the MEN1 Conditional Knockout Mouse: Behavioral Restoration and Gain of Function following the Reintroduction of MEN1 Gene.
    Article Snippet: The cells were permeabilized for 1 h with incubation medium (IM) containing 0.5% Triton and 10% goat serum in 1 × PBS.The cells were permeabilized for 1 h with incubation medium (IM) containing 0.5% Triton and 10% goat serum in 1 × PBS.. Primary antibody, menin C-terminal epitope (Bethyl Laboratories, A300-105, Montgomery, TX, USA), was used at 1:500 in IM for 1 h. Secondary antibodies, namely Alexa Fluor 488, 568, or 680 (Invitrogen, Vancouver, BC, Canada) were used at 1:100 in IM for 1 h followed by three 15 m washes in 1 × PBS.. Cells were mounted using ProLong Gold antifade reagent with DAPI (Invitrogen, BC, Canada).Cells were mounted using ProLong Gold antifade reagent with DAPI (Invitrogen, BC, Canada).

    Article Title: Selective Menin Deletion in the Hippocampal CA1 Region Leads to Disruption of Contextual Memory in the MEN1 Conditional Knockout Mouse: Behavioral Restoration and Gain of Function following the Reintroduction of MEN1 Gene.
    Article Snippet: The cells were permeabilized for 1 h with incubation medium (IM) containing 0.5% Triton and 10% goat serum in 1 × PBS.The cells were permeabilized for 1 h with incubation medium (IM) containing 0.5% Triton and 10% goat serum in 1 × PBS.. Primary antibody, menin C-terminal epitope [Bethyl Laboratories, A300-105, Montgomery, TX, USA], was used at 1:500 in IM for 1 h. Secondary antibodies, namely Alexa Fluor 488, 568, or 680 (Invitrogen, Vancouver, BC, Canada) were used at 1:100 in IM for 1 h followed by three 15 m washes in 1 × PBS.. Cells were mounted using ProLong Gold antifade reagent with DAPI (Invitrogen, BC, Canada).Cells were mounted using ProLong Gold antifade reagent with DAPI (Invitrogen, BC, Canada).

    Article Title: Selective Menin Deletion in the Hippocampal CA1 Region Leads to Disruption of Contextual Memory in the MEN1 Conditional Knockout Mouse: Behavioral Restoration and Gain of Function following the Reintroduction of MEN1 Gene
    Article Snippet: The cells were permeabilized for 1 h with incubation medium (IM) containing 0.5% Triton and 10% goat serum in 1 × PBS.The cells were permeabilized for 1 h with incubation medium (IM) containing 0.5% Triton and 10% goat serum in 1 × PBS.. Primary antibody, menin C-terminal epitope (Bethyl Laboratories, A300-105, Montgomery, TX, USA), was used at 1:500 in IM for 1 h. Secondary antibodies, namely Alexa Fluor 488, 568, or 680 (Invitrogen, Vancouver, BC, Canada) were used at 1:100 in IM for 1 h followed by three 15 m washes in 1 × PBS.. Cells were mounted using ProLong Gold antifade reagent with DAPI (Invitrogen, BC, Canada).Cells were mounted using ProLong Gold antifade reagent with DAPI (Invitrogen, BC, Canada).

    Control:

    Article Title: Selective Menin Deletion in the Hippocampal CA1 Region Leads to Disruption of Contextual Memory in the MEN1 Conditional Knockout Mouse: Behavioral Restoration and Gain of Function following the Reintroduction of MEN1 Gene.
    Article Snippet: The cells were permeabilized for 1 h with incubation medium (IM) containing 0.5% Triton and 10% goat serum in 1 × PBS.The cells were permeabilized for 1 h with incubation medium (IM) containing 0.5% Triton and 10% goat serum in 1 × PBS.. Primary antibody, menin C-terminal epitope (Bethyl Laboratories, A300-105, Montgomery, TX, USA), was used at 1:500 in IM for 1 h. Secondary antibodies, namely Alexa Fluor 488, 568, or 680 (Invitrogen, Vancouver, BC, Canada) were used at 1:100 in IM for 1 h followed by three 15 m washes in 1 × PBS.. Cells were mounted using ProLong Gold antifade reagent with DAPI (Invitrogen, BC, Canada).Cells were mounted using ProLong Gold antifade reagent with DAPI (Invitrogen, BC, Canada).

    Article Title: Selective Menin Deletion in the Hippocampal CA1 Region Leads to Disruption of Contextual Memory in the MEN1 Conditional Knockout Mouse: Behavioral Restoration and Gain of Function following the Reintroduction of MEN1 Gene.
    Article Snippet: The cells were permeabilized for 1 h with incubation medium (IM) containing 0.5% Triton and 10% goat serum in 1 × PBS.The cells were permeabilized for 1 h with incubation medium (IM) containing 0.5% Triton and 10% goat serum in 1 × PBS.. Primary antibody, menin C-terminal epitope [Bethyl Laboratories, A300-105, Montgomery, TX, USA], was used at 1:500 in IM for 1 h. Secondary antibodies, namely Alexa Fluor 488, 568, or 680 (Invitrogen, Vancouver, BC, Canada) were used at 1:100 in IM for 1 h followed by three 15 m washes in 1 × PBS.. Cells were mounted using ProLong Gold antifade reagent with DAPI (Invitrogen, BC, Canada).Cells were mounted using ProLong Gold antifade reagent with DAPI (Invitrogen, BC, Canada).

    Article Title: Selective Menin Deletion in the Hippocampal CA1 Region Leads to Disruption of Contextual Memory in the MEN1 Conditional Knockout Mouse: Behavioral Restoration and Gain of Function following the Reintroduction of MEN1 Gene
    Article Snippet: The cells were permeabilized for 1 h with incubation medium (IM) containing 0.5% Triton and 10% goat serum in 1 × PBS.The cells were permeabilized for 1 h with incubation medium (IM) containing 0.5% Triton and 10% goat serum in 1 × PBS.. Primary antibody, menin C-terminal epitope (Bethyl Laboratories, A300-105, Montgomery, TX, USA), was used at 1:500 in IM for 1 h. Secondary antibodies, namely Alexa Fluor 488, 568, or 680 (Invitrogen, Vancouver, BC, Canada) were used at 1:100 in IM for 1 h followed by three 15 m washes in 1 × PBS.. Cells were mounted using ProLong Gold antifade reagent with DAPI (Invitrogen, BC, Canada).Cells were mounted using ProLong Gold antifade reagent with DAPI (Invitrogen, BC, Canada).

    Molecular Weight:

    Article Title: Selective Menin Deletion in the Hippocampal CA1 Region Leads to Disruption of Contextual Memory in the MEN1 Conditional Knockout Mouse: Behavioral Restoration and Gain of Function following the Reintroduction of MEN1 Gene.
    Article Snippet: The cells were permeabilized for 1 h with incubation medium (IM) containing 0.5% Triton and 10% goat serum in 1 × PBS.The cells were permeabilized for 1 h with incubation medium (IM) containing 0.5% Triton and 10% goat serum in 1 × PBS.. Primary antibody, menin C-terminal epitope (Bethyl Laboratories, A300-105, Montgomery, TX, USA), was used at 1:500 in IM for 1 h. Secondary antibodies, namely Alexa Fluor 488, 568, or 680 (Invitrogen, Vancouver, BC, Canada) were used at 1:100 in IM for 1 h followed by three 15 m washes in 1 × PBS.. Cells were mounted using ProLong Gold antifade reagent with DAPI (Invitrogen, BC, Canada).Cells were mounted using ProLong Gold antifade reagent with DAPI (Invitrogen, BC, Canada).

    Article Title: Selective Menin Deletion in the Hippocampal CA1 Region Leads to Disruption of Contextual Memory in the MEN1 Conditional Knockout Mouse: Behavioral Restoration and Gain of Function following the Reintroduction of MEN1 Gene.
    Article Snippet: The cells were permeabilized for 1 h with incubation medium (IM) containing 0.5% Triton and 10% goat serum in 1 × PBS.The cells were permeabilized for 1 h with incubation medium (IM) containing 0.5% Triton and 10% goat serum in 1 × PBS.. Primary antibody, menin C-terminal epitope [Bethyl Laboratories, A300-105, Montgomery, TX, USA], was used at 1:500 in IM for 1 h. Secondary antibodies, namely Alexa Fluor 488, 568, or 680 (Invitrogen, Vancouver, BC, Canada) were used at 1:100 in IM for 1 h followed by three 15 m washes in 1 × PBS.. Cells were mounted using ProLong Gold antifade reagent with DAPI (Invitrogen, BC, Canada).Cells were mounted using ProLong Gold antifade reagent with DAPI (Invitrogen, BC, Canada).

    Article Title: Selective Menin Deletion in the Hippocampal CA1 Region Leads to Disruption of Contextual Memory in the MEN1 Conditional Knockout Mouse: Behavioral Restoration and Gain of Function following the Reintroduction of MEN1 Gene
    Article Snippet: The cells were permeabilized for 1 h with incubation medium (IM) containing 0.5% Triton and 10% goat serum in 1 × PBS.The cells were permeabilized for 1 h with incubation medium (IM) containing 0.5% Triton and 10% goat serum in 1 × PBS.. Primary antibody, menin C-terminal epitope (Bethyl Laboratories, A300-105, Montgomery, TX, USA), was used at 1:500 in IM for 1 h. Secondary antibodies, namely Alexa Fluor 488, 568, or 680 (Invitrogen, Vancouver, BC, Canada) were used at 1:100 in IM for 1 h followed by three 15 m washes in 1 × PBS.. Cells were mounted using ProLong Gold antifade reagent with DAPI (Invitrogen, BC, Canada).Cells were mounted using ProLong Gold antifade reagent with DAPI (Invitrogen, BC, Canada).

    Knock-Out:

    Article Title: Selective Menin Deletion in the Hippocampal CA1 Region Leads to Disruption of Contextual Memory in the MEN1 Conditional Knockout Mouse: Behavioral Restoration and Gain of Function following the Reintroduction of MEN1 Gene.
    Article Snippet: The cells were permeabilized for 1 h with incubation medium (IM) containing 0.5% Triton and 10% goat serum in 1 × PBS.The cells were permeabilized for 1 h with incubation medium (IM) containing 0.5% Triton and 10% goat serum in 1 × PBS.. Primary antibody, menin C-terminal epitope (Bethyl Laboratories, A300-105, Montgomery, TX, USA), was used at 1:500 in IM for 1 h. Secondary antibodies, namely Alexa Fluor 488, 568, or 680 (Invitrogen, Vancouver, BC, Canada) were used at 1:100 in IM for 1 h followed by three 15 m washes in 1 × PBS.. Cells were mounted using ProLong Gold antifade reagent with DAPI (Invitrogen, BC, Canada).Cells were mounted using ProLong Gold antifade reagent with DAPI (Invitrogen, BC, Canada).

    Article Title: Selective Menin Deletion in the Hippocampal CA1 Region Leads to Disruption of Contextual Memory in the MEN1 Conditional Knockout Mouse: Behavioral Restoration and Gain of Function following the Reintroduction of MEN1 Gene.
    Article Snippet: The cells were permeabilized for 1 h with incubation medium (IM) containing 0.5% Triton and 10% goat serum in 1 × PBS.The cells were permeabilized for 1 h with incubation medium (IM) containing 0.5% Triton and 10% goat serum in 1 × PBS.. Primary antibody, menin C-terminal epitope [Bethyl Laboratories, A300-105, Montgomery, TX, USA], was used at 1:500 in IM for 1 h. Secondary antibodies, namely Alexa Fluor 488, 568, or 680 (Invitrogen, Vancouver, BC, Canada) were used at 1:100 in IM for 1 h followed by three 15 m washes in 1 × PBS.. Cells were mounted using ProLong Gold antifade reagent with DAPI (Invitrogen, BC, Canada).Cells were mounted using ProLong Gold antifade reagent with DAPI (Invitrogen, BC, Canada).

    Article Title: Selective Menin Deletion in the Hippocampal CA1 Region Leads to Disruption of Contextual Memory in the MEN1 Conditional Knockout Mouse: Behavioral Restoration and Gain of Function following the Reintroduction of MEN1 Gene
    Article Snippet: The cells were permeabilized for 1 h with incubation medium (IM) containing 0.5% Triton and 10% goat serum in 1 × PBS.The cells were permeabilized for 1 h with incubation medium (IM) containing 0.5% Triton and 10% goat serum in 1 × PBS.. Primary antibody, menin C-terminal epitope (Bethyl Laboratories, A300-105, Montgomery, TX, USA), was used at 1:500 in IM for 1 h. Secondary antibodies, namely Alexa Fluor 488, 568, or 680 (Invitrogen, Vancouver, BC, Canada) were used at 1:100 in IM for 1 h followed by three 15 m washes in 1 × PBS.. Cells were mounted using ProLong Gold antifade reagent with DAPI (Invitrogen, BC, Canada).Cells were mounted using ProLong Gold antifade reagent with DAPI (Invitrogen, BC, Canada).

    Comparison:

    Article Title: Selective Menin Deletion in the Hippocampal CA1 Region Leads to Disruption of Contextual Memory in the MEN1 Conditional Knockout Mouse: Behavioral Restoration and Gain of Function following the Reintroduction of MEN1 Gene.
    Article Snippet: The cells were permeabilized for 1 h with incubation medium (IM) containing 0.5% Triton and 10% goat serum in 1 × PBS.The cells were permeabilized for 1 h with incubation medium (IM) containing 0.5% Triton and 10% goat serum in 1 × PBS.. Primary antibody, menin C-terminal epitope (Bethyl Laboratories, A300-105, Montgomery, TX, USA), was used at 1:500 in IM for 1 h. Secondary antibodies, namely Alexa Fluor 488, 568, or 680 (Invitrogen, Vancouver, BC, Canada) were used at 1:100 in IM for 1 h followed by three 15 m washes in 1 × PBS.. Cells were mounted using ProLong Gold antifade reagent with DAPI (Invitrogen, BC, Canada).Cells were mounted using ProLong Gold antifade reagent with DAPI (Invitrogen, BC, Canada).

    Article Title: Selective Menin Deletion in the Hippocampal CA1 Region Leads to Disruption of Contextual Memory in the MEN1 Conditional Knockout Mouse: Behavioral Restoration and Gain of Function following the Reintroduction of MEN1 Gene.
    Article Snippet: The cells were permeabilized for 1 h with incubation medium (IM) containing 0.5% Triton and 10% goat serum in 1 × PBS.The cells were permeabilized for 1 h with incubation medium (IM) containing 0.5% Triton and 10% goat serum in 1 × PBS.. Primary antibody, menin C-terminal epitope [Bethyl Laboratories, A300-105, Montgomery, TX, USA], was used at 1:500 in IM for 1 h. Secondary antibodies, namely Alexa Fluor 488, 568, or 680 (Invitrogen, Vancouver, BC, Canada) were used at 1:100 in IM for 1 h followed by three 15 m washes in 1 × PBS.. Cells were mounted using ProLong Gold antifade reagent with DAPI (Invitrogen, BC, Canada).Cells were mounted using ProLong Gold antifade reagent with DAPI (Invitrogen, BC, Canada).

    Article Title: Selective Menin Deletion in the Hippocampal CA1 Region Leads to Disruption of Contextual Memory in the MEN1 Conditional Knockout Mouse: Behavioral Restoration and Gain of Function following the Reintroduction of MEN1 Gene
    Article Snippet: The cells were permeabilized for 1 h with incubation medium (IM) containing 0.5% Triton and 10% goat serum in 1 × PBS.The cells were permeabilized for 1 h with incubation medium (IM) containing 0.5% Triton and 10% goat serum in 1 × PBS.. Primary antibody, menin C-terminal epitope (Bethyl Laboratories, A300-105, Montgomery, TX, USA), was used at 1:500 in IM for 1 h. Secondary antibodies, namely Alexa Fluor 488, 568, or 680 (Invitrogen, Vancouver, BC, Canada) were used at 1:100 in IM for 1 h followed by three 15 m washes in 1 × PBS.. Cells were mounted using ProLong Gold antifade reagent with DAPI (Invitrogen, BC, Canada).Cells were mounted using ProLong Gold antifade reagent with DAPI (Invitrogen, BC, Canada).

    MANN-WHITNEY:

    Article Title: Selective Menin Deletion in the Hippocampal CA1 Region Leads to Disruption of Contextual Memory in the MEN1 Conditional Knockout Mouse: Behavioral Restoration and Gain of Function following the Reintroduction of MEN1 Gene.
    Article Snippet: The cells were permeabilized for 1 h with incubation medium (IM) containing 0.5% Triton and 10% goat serum in 1 × PBS.The cells were permeabilized for 1 h with incubation medium (IM) containing 0.5% Triton and 10% goat serum in 1 × PBS.. Primary antibody, menin C-terminal epitope (Bethyl Laboratories, A300-105, Montgomery, TX, USA), was used at 1:500 in IM for 1 h. Secondary antibodies, namely Alexa Fluor 488, 568, or 680 (Invitrogen, Vancouver, BC, Canada) were used at 1:100 in IM for 1 h followed by three 15 m washes in 1 × PBS.. Cells were mounted using ProLong Gold antifade reagent with DAPI (Invitrogen, BC, Canada).Cells were mounted using ProLong Gold antifade reagent with DAPI (Invitrogen, BC, Canada).

    Article Title: Selective Menin Deletion in the Hippocampal CA1 Region Leads to Disruption of Contextual Memory in the MEN1 Conditional Knockout Mouse: Behavioral Restoration and Gain of Function following the Reintroduction of MEN1 Gene.
    Article Snippet: The cells were permeabilized for 1 h with incubation medium (IM) containing 0.5% Triton and 10% goat serum in 1 × PBS.The cells were permeabilized for 1 h with incubation medium (IM) containing 0.5% Triton and 10% goat serum in 1 × PBS.. Primary antibody, menin C-terminal epitope [Bethyl Laboratories, A300-105, Montgomery, TX, USA], was used at 1:500 in IM for 1 h. Secondary antibodies, namely Alexa Fluor 488, 568, or 680 (Invitrogen, Vancouver, BC, Canada) were used at 1:100 in IM for 1 h followed by three 15 m washes in 1 × PBS.. Cells were mounted using ProLong Gold antifade reagent with DAPI (Invitrogen, BC, Canada).Cells were mounted using ProLong Gold antifade reagent with DAPI (Invitrogen, BC, Canada).

    Article Title: Selective Menin Deletion in the Hippocampal CA1 Region Leads to Disruption of Contextual Memory in the MEN1 Conditional Knockout Mouse: Behavioral Restoration and Gain of Function following the Reintroduction of MEN1 Gene
    Article Snippet: The cells were permeabilized for 1 h with incubation medium (IM) containing 0.5% Triton and 10% goat serum in 1 × PBS.The cells were permeabilized for 1 h with incubation medium (IM) containing 0.5% Triton and 10% goat serum in 1 × PBS.. Primary antibody, menin C-terminal epitope (Bethyl Laboratories, A300-105, Montgomery, TX, USA), was used at 1:500 in IM for 1 h. Secondary antibodies, namely Alexa Fluor 488, 568, or 680 (Invitrogen, Vancouver, BC, Canada) were used at 1:100 in IM for 1 h followed by three 15 m washes in 1 × PBS.. Cells were mounted using ProLong Gold antifade reagent with DAPI (Invitrogen, BC, Canada).Cells were mounted using ProLong Gold antifade reagent with DAPI (Invitrogen, BC, Canada).

    Fluorescence:

    Article Title: Selective Menin Deletion in the Hippocampal CA1 Region Leads to Disruption of Contextual Memory in the MEN1 Conditional Knockout Mouse: Behavioral Restoration and Gain of Function following the Reintroduction of MEN1 Gene.
    Article Snippet: The cells were permeabilized for 1 h with incubation medium (IM) containing 0.5% Triton and 10% goat serum in 1 × PBS.The cells were permeabilized for 1 h with incubation medium (IM) containing 0.5% Triton and 10% goat serum in 1 × PBS.. Primary antibody, menin C-terminal epitope (Bethyl Laboratories, A300-105, Montgomery, TX, USA), was used at 1:500 in IM for 1 h. Secondary antibodies, namely Alexa Fluor 488, 568, or 680 (Invitrogen, Vancouver, BC, Canada) were used at 1:100 in IM for 1 h followed by three 15 m washes in 1 × PBS.. Cells were mounted using ProLong Gold antifade reagent with DAPI (Invitrogen, BC, Canada).Cells were mounted using ProLong Gold antifade reagent with DAPI (Invitrogen, BC, Canada).

    Article Title: Selective Menin Deletion in the Hippocampal CA1 Region Leads to Disruption of Contextual Memory in the MEN1 Conditional Knockout Mouse: Behavioral Restoration and Gain of Function following the Reintroduction of MEN1 Gene.
    Article Snippet: The cells were permeabilized for 1 h with incubation medium (IM) containing 0.5% Triton and 10% goat serum in 1 × PBS.The cells were permeabilized for 1 h with incubation medium (IM) containing 0.5% Triton and 10% goat serum in 1 × PBS.. Primary antibody, menin C-terminal epitope [Bethyl Laboratories, A300-105, Montgomery, TX, USA], was used at 1:500 in IM for 1 h. Secondary antibodies, namely Alexa Fluor 488, 568, or 680 (Invitrogen, Vancouver, BC, Canada) were used at 1:100 in IM for 1 h followed by three 15 m washes in 1 × PBS.. Cells were mounted using ProLong Gold antifade reagent with DAPI (Invitrogen, BC, Canada).Cells were mounted using ProLong Gold antifade reagent with DAPI (Invitrogen, BC, Canada).

    Article Title: Selective Menin Deletion in the Hippocampal CA1 Region Leads to Disruption of Contextual Memory in the MEN1 Conditional Knockout Mouse: Behavioral Restoration and Gain of Function following the Reintroduction of MEN1 Gene
    Article Snippet: The cells were permeabilized for 1 h with incubation medium (IM) containing 0.5% Triton and 10% goat serum in 1 × PBS.The cells were permeabilized for 1 h with incubation medium (IM) containing 0.5% Triton and 10% goat serum in 1 × PBS.. Primary antibody, menin C-terminal epitope (Bethyl Laboratories, A300-105, Montgomery, TX, USA), was used at 1:500 in IM for 1 h. Secondary antibodies, namely Alexa Fluor 488, 568, or 680 (Invitrogen, Vancouver, BC, Canada) were used at 1:100 in IM for 1 h followed by three 15 m washes in 1 × PBS.. Cells were mounted using ProLong Gold antifade reagent with DAPI (Invitrogen, BC, Canada).Cells were mounted using ProLong Gold antifade reagent with DAPI (Invitrogen, BC, Canada).



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    Figure 9. Reduced C-menin expression in MEN1 CKO post-behavior testing. (A) WB of the mouse hippocampal protein samples with β-Tubulin antibody (loading control) showing the expression of β-Tubulin in controls, and CKO showing relatively similar fluorescence across the controls and CKO at the expected molecular weight of 50 kDA; (B) WB of the mouse hippocampal protein samples with menin C-terminal <t>epitope</t> antibody for the detection of the C-terminal fragment in the controls (C) and the conditional knockout (CKO) animals at the expected molecular weight of 20 kDA, and the bands from the control animals have higher fluorescence compared to the CKOs; (C) summary data, showing the comparison of menin-to-β-Tubulin ratio in controls and the CKOs, with a reduction in the C-menin-to-β-Tubulin ratio in the CKO. Data represent the mean ratio ±SEM. n = 4. Statistical significance (Mann–Whitney U test), ** p < 0.05.
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    Image Search Results


    Figure 9. Reduced C-menin expression in MEN1 CKO post-behavior testing. (A) WB of the mouse hippocampal protein samples with β-Tubulin antibody (loading control) showing the expression of β-Tubulin in controls, and CKO showing relatively similar fluorescence across the controls and CKO at the expected molecular weight of 50 kDA; (B) WB of the mouse hippocampal protein samples with menin C-terminal epitope antibody for the detection of the C-terminal fragment in the controls (C) and the conditional knockout (CKO) animals at the expected molecular weight of 20 kDA, and the bands from the control animals have higher fluorescence compared to the CKOs; (C) summary data, showing the comparison of menin-to-β-Tubulin ratio in controls and the CKOs, with a reduction in the C-menin-to-β-Tubulin ratio in the CKO. Data represent the mean ratio ±SEM. n = 4. Statistical significance (Mann–Whitney U test), ** p < 0.05.

    Journal: Cells

    Article Title: Selective Menin Deletion in the Hippocampal CA1 Region Leads to Disruption of Contextual Memory in the MEN1 Conditional Knockout Mouse: Behavioral Restoration and Gain of Function following the Reintroduction of MEN1 Gene.

    doi: 10.3390/cells11244019

    Figure Lengend Snippet: Figure 9. Reduced C-menin expression in MEN1 CKO post-behavior testing. (A) WB of the mouse hippocampal protein samples with β-Tubulin antibody (loading control) showing the expression of β-Tubulin in controls, and CKO showing relatively similar fluorescence across the controls and CKO at the expected molecular weight of 50 kDA; (B) WB of the mouse hippocampal protein samples with menin C-terminal epitope antibody for the detection of the C-terminal fragment in the controls (C) and the conditional knockout (CKO) animals at the expected molecular weight of 20 kDA, and the bands from the control animals have higher fluorescence compared to the CKOs; (C) summary data, showing the comparison of menin-to-β-Tubulin ratio in controls and the CKOs, with a reduction in the C-menin-to-β-Tubulin ratio in the CKO. Data represent the mean ratio ±SEM. n = 4. Statistical significance (Mann–Whitney U test), ** p < 0.05.

    Article Snippet: Primary antibody, menin C-terminal epitope (Bethyl Laboratories, A300-105, Montgomery, TX, USA), was used at 1:500 in IM for 1 h. Secondary antibodies, namely Alexa Fluor 488, 568, or 680 (Invitrogen, Vancouver, BC, Canada) were used at 1:100 in IM for 1 h followed by three 15 m washes in 1 × PBS.

    Techniques: Expressing, Control, Molecular Weight, Knock-Out, Comparison, MANN-WHITNEY

    Reduced menin expression in the hippocampal sections of the CKO animals. ( A ) Hippocampal section of the control mice injected with the control virus; ( i ) DAPI for nuclear staining; ( ii ) GFP-expressing neurons after the transduction of the control virus ( iii ). C-menin’s expression in the neurons ( iv ). Merge for showing the overlap between GFP-expressing neurons, DAPI, and C-menin; ( B ) hippocampal section of the CKO mice injected with the Cre-expressing virus; ( i ) DAPI for nuclear staining; ( ii ) GFP-expressing neurons after the transduction of the Cre recombinase-inducing virus; ( iii ) C-menin’s weak signal in the neurons; ( iv ) merge for showing the overlap between GFP expression, DAPI, and C-menin; ( C ) summary data, showing the reduction in menin’s signal as measured by mean gray-to-area ratio in the CKO compared to the controls. Data represent the mean ratio ± SEM. n = 4. Statistical significance (Mann–Whitney U test), * p < 0.05.

    Journal: Cells

    Article Title: Selective Menin Deletion in the Hippocampal CA1 Region Leads to Disruption of Contextual Memory in the MEN1 Conditional Knockout Mouse: Behavioral Restoration and Gain of Function following the Reintroduction of MEN1 Gene

    doi: 10.3390/cells11244019

    Figure Lengend Snippet: Reduced menin expression in the hippocampal sections of the CKO animals. ( A ) Hippocampal section of the control mice injected with the control virus; ( i ) DAPI for nuclear staining; ( ii ) GFP-expressing neurons after the transduction of the control virus ( iii ). C-menin’s expression in the neurons ( iv ). Merge for showing the overlap between GFP-expressing neurons, DAPI, and C-menin; ( B ) hippocampal section of the CKO mice injected with the Cre-expressing virus; ( i ) DAPI for nuclear staining; ( ii ) GFP-expressing neurons after the transduction of the Cre recombinase-inducing virus; ( iii ) C-menin’s weak signal in the neurons; ( iv ) merge for showing the overlap between GFP expression, DAPI, and C-menin; ( C ) summary data, showing the reduction in menin’s signal as measured by mean gray-to-area ratio in the CKO compared to the controls. Data represent the mean ratio ± SEM. n = 4. Statistical significance (Mann–Whitney U test), * p < 0.05.

    Article Snippet: Primary antibody, menin C-terminal epitope [Bethyl Laboratories, A300-105, Montgomery, TX, USA], was used at 1:500 in IM for 1 h. Secondary antibodies, namely Alexa Fluor 488, 568, or 680 (Invitrogen, Vancouver, BC, Canada) were used at 1:100 in IM for 1 h followed by three 15 m washes in 1 × PBS.

    Techniques: Expressing, Injection, Staining, Transduction, MANN-WHITNEY

    Reduced C-menin expression in MEN1 CKO post-behavior testing. ( A ) WB of the mouse hippocampal protein samples with β-Tubulin antibody (loading control) showing the expression of β-Tubulin in controls, and CKO showing relatively similar fluorescence across the controls and CKO at the expected molecular weight of 50 kDA; ( B ) WB of the mouse hippocampal protein samples with menin C-terminal epitope antibody for the detection of the C-terminal fragment in the controls ( C ) and the conditional knockout (CKO) animals at the expected molecular weight of 20 kDA, and the bands from the control animals have higher fluorescence compared to the CKOs; ( C ) summary data, showing the comparison of menin-to-β-Tubulin ratio in controls and the CKOs, with a reduction in the C-menin-to-β-Tubulin ratio in the CKO. Data represent the mean ratio ±SEM. n = 4. Statistical significance (Mann–Whitney U test), ** p < 0.05.

    Journal: Cells

    Article Title: Selective Menin Deletion in the Hippocampal CA1 Region Leads to Disruption of Contextual Memory in the MEN1 Conditional Knockout Mouse: Behavioral Restoration and Gain of Function following the Reintroduction of MEN1 Gene

    doi: 10.3390/cells11244019

    Figure Lengend Snippet: Reduced C-menin expression in MEN1 CKO post-behavior testing. ( A ) WB of the mouse hippocampal protein samples with β-Tubulin antibody (loading control) showing the expression of β-Tubulin in controls, and CKO showing relatively similar fluorescence across the controls and CKO at the expected molecular weight of 50 kDA; ( B ) WB of the mouse hippocampal protein samples with menin C-terminal epitope antibody for the detection of the C-terminal fragment in the controls ( C ) and the conditional knockout (CKO) animals at the expected molecular weight of 20 kDA, and the bands from the control animals have higher fluorescence compared to the CKOs; ( C ) summary data, showing the comparison of menin-to-β-Tubulin ratio in controls and the CKOs, with a reduction in the C-menin-to-β-Tubulin ratio in the CKO. Data represent the mean ratio ±SEM. n = 4. Statistical significance (Mann–Whitney U test), ** p < 0.05.

    Article Snippet: Primary antibody, menin C-terminal epitope [Bethyl Laboratories, A300-105, Montgomery, TX, USA], was used at 1:500 in IM for 1 h. Secondary antibodies, namely Alexa Fluor 488, 568, or 680 (Invitrogen, Vancouver, BC, Canada) were used at 1:100 in IM for 1 h followed by three 15 m washes in 1 × PBS.

    Techniques: Expressing, Fluorescence, Molecular Weight, Knock-Out, MANN-WHITNEY

    In vitro expression of MEN1 rescue virus, pAAV-hSyn-HI-eGFP-MEN1, in the hippocampal cultured neurons. ( A ) The GFP-expressing neurons after the transduction of the MEN1 rescue virus; ( i ) the GFP-expressing neurons; ( ii ) DAPI for nuclear staining; ( iii ) C-menin expression in the neurons; ( iv ) merge for showing the overlap between GFP-expressing neurons, DAPI, and C-menin; ( B ) the non-GFP expressing regions (controls); ( i ) the non-GFP-expressing neurons; ( ii ) DAPI for nuclear staining; ( iii ) C-menin expression in the neurons; ( iv ) merge for showing the overlap between GFP expression, DAPI, and C-menin; ( C ) summary data, showing the mean gray-to-area ratio for Cy5 channel for C-menin in GFP expressing and non-GFP expressing regions, indicating an increase in C-menin expression in GFP-expressing neurons. Data represent the mean ± SEM, n = 4, Statistical significance (Mann–Whitney U test), ** p < 0.05.

    Journal: Cells

    Article Title: Selective Menin Deletion in the Hippocampal CA1 Region Leads to Disruption of Contextual Memory in the MEN1 Conditional Knockout Mouse: Behavioral Restoration and Gain of Function following the Reintroduction of MEN1 Gene

    doi: 10.3390/cells11244019

    Figure Lengend Snippet: In vitro expression of MEN1 rescue virus, pAAV-hSyn-HI-eGFP-MEN1, in the hippocampal cultured neurons. ( A ) The GFP-expressing neurons after the transduction of the MEN1 rescue virus; ( i ) the GFP-expressing neurons; ( ii ) DAPI for nuclear staining; ( iii ) C-menin expression in the neurons; ( iv ) merge for showing the overlap between GFP-expressing neurons, DAPI, and C-menin; ( B ) the non-GFP expressing regions (controls); ( i ) the non-GFP-expressing neurons; ( ii ) DAPI for nuclear staining; ( iii ) C-menin expression in the neurons; ( iv ) merge for showing the overlap between GFP expression, DAPI, and C-menin; ( C ) summary data, showing the mean gray-to-area ratio for Cy5 channel for C-menin in GFP expressing and non-GFP expressing regions, indicating an increase in C-menin expression in GFP-expressing neurons. Data represent the mean ± SEM, n = 4, Statistical significance (Mann–Whitney U test), ** p < 0.05.

    Article Snippet: Primary antibody, menin C-terminal epitope [Bethyl Laboratories, A300-105, Montgomery, TX, USA], was used at 1:500 in IM for 1 h. Secondary antibodies, namely Alexa Fluor 488, 568, or 680 (Invitrogen, Vancouver, BC, Canada) were used at 1:100 in IM for 1 h followed by three 15 m washes in 1 × PBS.

    Techniques: In Vitro, Expressing, Cell Culture, Transduction, Staining, MANN-WHITNEY